Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SUMO2

Cell type

Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
acute myeloid leukemia
cell line
HL-60
treatment
mock
chip antibody
SUMO2/3
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM5962056
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from fixed HL-60 (18 milions cells) were clarified for nuclei, then chromatin was sonicated and SUMO2/3-DNA complexes were isolated with SUMO2/3 antibody (2µg per IP). Three biological replicates were performed and pooled for sequencing. Libraries were prepared according to Illumina's instructions TruSeq®ChIP Sample Preparation (Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
50671857
Reads aligned (%)
97.5
Duplicates removed (%)
11.2
Number of peaks
1521 (qval < 1E-05)

hg19

Number of total reads
50671857
Reads aligned (%)
96.5
Duplicates removed (%)
13.0
Number of peaks
1458 (qval < 1E-05)

Base call quality data from DBCLS SRA